Neutrophil Cathepsin G, but Not Elastase, Induces Aggregation of MCF-7 Mammary Carcinoma Cells by a Protease Activity-Dependent Cell-Oriented Mechanism
Figure 6
Induction of MCF-7 cell aggregation by cathepsin G, but not elastase, treatment of PLL-coated wells. (a) PLL-coated wells were treated overnight with cathepsin G () or elastase (●). After the enzymes were removed by washing, MCF-7 cells suspended in 1% BSA-medium were seeded and incubated for an additional 24 h. The cell aggregation index was evaluated as described in the legend of Figure 2. The results are expressed as the mean (). When the bars are not shown, they are smaller than the size of the symbols. Original magnification: ×100. (b) PLL-coated wells were treated overnight with cathepsin G for 24 h, and then the wells were washed and treated without (open column) or with PMSF (4 mM, filled column) at 37°C for 1 h. MCF-7 cells suspended in 1% BSA-medium were then seeded and incubated for an additional 24 h. Cell aggregation was evaluated as above. Lower photos: images of MCF-7 cells at 24 h after cultivation in the cathepsin G (2.5 nM)-treated PLL-coated wells, subsequently treated without (left) or with (right) PMSF.
