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Mediators of Inflammation
Volume 2014 (2014), Article ID 975061, 7 pages
http://dx.doi.org/10.1155/2014/975061
Research Article

Sequential Analysis of Oxidative Stress Markers and Vitamin C Status in Acute Bacterial Osteomyelitis

1Clinics for General and Orthopedic Surgery, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia
2Institute of Biochemistry, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia
3Institute of Pathophysiology, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia
4Institute of Physiology, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia

Received 15 June 2014; Accepted 28 July 2014; Published 10 August 2014

Academic Editor: Yung-Hsiang Chen

Copyright © 2014 Rade Grbic et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In bacterial bone infections, excessively formed oxidants may result in local and systemic oxidative stress. Vitamin C is the major extracellular nonenzymatic antioxidant, also implicated in bone cells metabolism and viability. The physiological functions of vitamin C largely depend on its redox status. We sequentially assessed oxidative stress markers, hydroperoxides and malondialdehyde (MDA), total antioxidant activity (AOA), total vitamin C, ascorbic acid (Asc), and oxidized/reduced vitamin C ratio in 137 patients with acute osteomyelitis (OM). Compared to 52 healthy controls, in OM group baseline serum hydroperoxides, MDA and oxidized/reduced vitamin C ratio were higher whilst Asc and AOA were lower (, resp.). On the other side, total vitamin C levels in patients and controls were similar , thereby suggesting a relative rather than absolute vitamin C deficiency in OM. During the follow-up, oxidative stress markers, AOA, and oxidizedreduced vitamin C ratio were gradually returned to normal, while there was no apparent change of total vitamin C concentrations. Persistently high values of oxidized/reduced vitamin C ratio and serum MDA were found in subacute OM. In conclusion, acute OM was associated with enhanced systemic oxidative stress and the shift of vitamin C redox status towards oxidized forms.