LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis

Figure 3

Catalase (CAT) in PDL cells. (a) CAT was visualized by immunofluorescence staining (1 : 50; antibody from Abcam). PDL cells were cultured under normoxic (Nox) or hypoxic condition (Hox) and stimulated with or without LPS of Porphyromonas gingivalis (1 μg/mL). Catalase cytoplasm density (b) was determined in relation to cell area using the freely available image-processing software ImageJ 1.43 (http://rsb.info.nih.gov/ij/). Statistical analysis of immunofluorescence data was analyzed by one-way ANOVA and post hoc Dunnett and Tukey’s multiple comparison test; ^# difference to control; * difference between groups (means SD; ). The scale bars indicate 100 μm in the immunofluorescence images.

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