Jussara (Euterpe edulis Mart.) Supplementation during Pregnancy and Lactation Modulates the Gene and Protein Expression of Inflammation Biomarkers Induced by trans-Fatty Acids in the Colon of Offspring

Table 2

Nucleotide sequence of the forward and reverse primers for the RT-PCR.

Target mRNA	Forward primer	Reverse primer
HPRT	5′-CTCATGGACTGATTATGGACAGGA-3′	5′-GCAGGTCAGCAAAGAACTTATAGC-3′
TLR-4	5′-GCATCATCTTCATTGTCCTTGAGA-3′	5′-CTACCTTTTCGGAACTTAGGTCTACT-3′
TNF-R	5′-GAA CAC CGT GTG TAA CTG CC-3′	5′-ATT CCT TCA CCC TCC ACC TC-3′
IL-6R	5′ AAGCAGGTCCAGCCACAATGTAG 3′	5′ CCAACTGACTTTGAGCCAACGAG 3′
All bacteria	5′-TCC TAC GGG AGG CAG CAG T-3′	5′-GAC TAC CAG GGT ATC TAA TCC TGT T-3′
Lactobacillus spp.	5′-AGC AGT AGG GAA TCT TCC A-3′	5′-CAC CGC TAC ACA TGG AG-3′