3,5,4′-Tri-O-acetylresveratrol Attenuates Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome via MAPK/SIRT1 Pathway

Figure 8

Neutralizing effects of EX527 on the protecting effects of Rsv on LPS stimulated NR8383 cells. (a) Expression of SIRT1 was evaluated by western blot and normalized to the expression level of β-actin. LPS stimulation inhibited the expression of SIRT1 compared with that of control, versus control, while Rsv treatment significantly reversed the inhibiting effects, versus ; on the contrary, EX527 and Rsv cotreatment counterbalanced the enhancing effects of Rsv on the expression of SIRT1, versus . (b–d) Contents of TNF-α, IL-6, and IL-1β were evaluated by ELISA. LPS stimulation increased the generation of TNF-α, IL-6, and IL-1β in NR8383 cells compared with that of control, versus control, while Rsv treatment significantly inhibited the formation of those cytokines in NR8383 cells when stimulated by LPS, versus ; astonishingly, EX527 and Rsv cotreatment neutralized the inhibiting effects of Rsv on the generation of TNF-α, IL-6, and IL-1β. versus .

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