Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 2015, Article ID 161579, 7 pages
http://dx.doi.org/10.1155/2015/161579
Research Article

T, B, and NKT Cells in Systemic Inflammation in Obstructive Sleep Apnoea

1Department of Pneumonology and Allergology, Medical University of Warsaw, Ulica Banacha 1a, 02 097 Warsaw, Poland
2Laboratory of Flow Cytometry, Medical Centre of Postgraduate Education, Ulica Marymoncka 99, 01 813 Warsaw, Poland

Received 12 January 2015; Revised 12 May 2015; Accepted 12 May 2015

Academic Editor: Tânia Silvia Fröde

Copyright © 2015 Joanna Domagała-Kulawik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Obstructive sleep apnoea syndrome (OSAS) brings risk of serious complications. The study objective was to assess elements of the cellular immune response in the course of OSAS. Methods. Peripheral blood (PB) lymphocytes: T, B, NK, NKT-like, Th, Tc, and HLA DR+ T cells were evaluated by flow cytometry of 48 OSA patients; the concentration of adiponectin, interleukin 1β, and TNFα was measured by ELISA method. The OSA complication score was developed and used for statistical analysis. Results. The proportion of B cells and Th/Tc ratio were significantly lower in the BP of OSA patients when compared with control subjects (median 7.9 versus 10.9%, 0.9 versus 1.5, ). The proportion of Tc, NK, NKT-like, and HLADR positive T cells were elevated in OSA patients when compared with healthy subjects (36.4 versus 26.8, 15.5 versus 8.5, 5.7 versus 3.0, and 8.4 versus 4.5%, , resp.) and were more pronounced in patients with metabolic syndrome. The grade of OSA complication score correlated with systemic inflammation markers and the proportion of B cells. The value of adiponectin/BMI ratio correlated significantly with SpO2 (, ), CRP (, ), TNFα concentration (, ), and proportion of B cells (, ). Conclusion. Lymphocytes B, Tc, NK, NKT-like, and adiponectin are involved in systemic immune response in OSA patients possibly predisposing them to cardiovascular and metabolic complications.