Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-κB Nuclear Translocation and Suppression of Experimental Asthma in Mice

Figure 6

Effect of DMPF-1 on TNF-α-stimulated phosphorylation of MAP kinases in A549 cells. Cells were treated with DMPF-1 (7.5 μM) or positive controls in the presence or absence of TNF-α for 30 min. Whole cell protein extract was subjected to Western blot analysis. Expression levels of phosphorylated protein of (a) p38, (b) JNK, and (c) ERK were quantified and normalized to nonphosphorylated proteins. The values are expressed as mean ± SEM of three independent experiments. and , significantly different from the TNF-α-stimulated control group; SB: SB203580; SP: SP600125; PD: PD98059.

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