Research Article

Cyclic GMP-AMP Synthase Is Required for Cell Proliferation and Inflammatory Responses in Rheumatoid Arthritis Synoviocytes

Figure 2

The expression of cGAS gene in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) was inhibited by tumor necrosis factor α (TNFα) inflammatory stimulation. (a) cGAS mRNA was upregulated by TNFα stimulation in a time-dependent manner. RA FLS (2 × 105) were cultured in 6-well plates and then treated with TNFα (100 ng/mL) for different times (0, 15, 30, 60, and 120 min). (b) The level of cGAS protein was determined by Western blot. Values are the mean and SEM (standard error of the mean). Results are representative of 3 independent experiments. versus 0 min.
(a)
(b)