Identification of Anti-Long Chain Saturated Fatty Acid IgG Antibodies in Serum of Patients with Type 2 Diabetes

Figure 6

Serum nonesterified serum FA. (a) Bar graph of serum nonesterified FA among diabetic and nondiabetic cohorts. CC = Caucasian control; CD = Caucasian diabetic; HC = Hispanic control; HD: Hispanic diabetic. Data was analyzed by Mann-Whitney. , . (b) plot of anti-LCSFA IgG and nonesterified serum FA for all serum samples in this study. (c) Anti-LCSFA IgG neutralizes PA-induced IL-β secretion from DCs in the presence of BSA. Monocyte derived dendritic cells from healthy patients were cultured with 150 μM PA (in 1 : 1 ratio with BSA) in the presence of a mixture of IgG antibody isolated from 5 En Balance participants who tested positive for anti-LCSFA IgG (black bars) or in the presence of a mixture of IgG antibody isolated from 2 En Balance participants who tested negative for the antibody after the 3-month intervention (white bars). PA + Ab (pre) indicates dendritic cells that were treated with PA preabsorbed for 2 hrs with total IgG from the two groups described. After 24 hrs, media from the cell cultures were analyzed by cytometric bead array for IL-1β. Data was analyzed by one-way ANOVA. . (d) Illustration of the potential mechanism for the generation of anti-LCSFA IgG.

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