CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis

Table 1

Main characteristics of the model.

	ND ()	HFD ()
weight (g)	5.6 (3–9)	30.6 (12–42)	0.001
Fasting glucose (mg/dL)	101 (85–148)	166 (77–291)	0.02
ALT (UI/L)	30 (20–33)	223 (179–250)	0.01
NAS	0	4.5 (1–6)	0.000004
Steatosis ()
0	7	1
1	0	4
2	0	3	0.000451
Lobular inflammation ()
0	7	0
1	0	6
2	0	2
3	0	0	0.00002
Ballooning ()
0	7	1
1	0	0
2	0	7	0.000008
Fibrosis ()
0	7	5
1	0	3
2	0	0	0.08

Validation of the model: weight (weight at sacrifice (g) − weight baseline (g)), fasting glucose levels (mg/dL) and histological features (NASH Activity Score (NAS) calculated as the sum of the subscores for steatosis, lobular inflammation, and ballooning) (: number of mice per score). Data are expressed as median and range or as number. ALT: alanine aminotransferase; ND: normal diet; HFD: high fat diet; : -value of the comparison between ND and HFD (Mann-Whitney test and Student’s -test, as appropriate). Statistically significant.