Claudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization

Figure 4

Claudin-5 surface localization on blood vessel endothelial cells does not change over time. Claudin-5 is absent from the surface of jejunal enterocytes in 24-hour-old ((a), (b)) and 6-week-old piglets ((e), (f)), respectively but it is present on the blood vessel walls in the submucosa for both age groups ((c), (d), (g), and (h)). Red cells within villi are from autofluorescent cells and red cells within the blood vessels are red blood cells. These images are representatives of IHC performed on tissue from 3 animals per time point. Primary antibody: rabbit anti-Claudin-5. Secondary antibody: PE-labeled goat anti-rabbit (red). Nuclear stain: DAPI (blue). Scale bar represents 40 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)