Claudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization

Table 1

Primer information.

Gene	Source	Sequence (5′-3′)	Amplicon size	Annealing temperature
ACTB	Nygard et al., 2007 [49]	Forward: CACGCCATCCTGCGTCTGGA; Reverse: AGCACCGTGTTGGCGTAGAG	108	60
CLDN4	NM_001161637.1; (NCBI)	Forward: CAACTGCGTGGATGATGAGA; Reverse: CCAGGGGATTGTAGAAGTCG	140	60
CLDN5	NM_001161636.1; (NCBI)	Forward: CCTTCCTGGACCACAACATC; Reverse: CACCGAGTCGTACACCTTGC	110	60
FCGRT	NM_214197.2; (NCBI)	Forward: GTCTGGGAAAGCCAGGTGT; Reverse: CCTCCTTCCTCCAAGGTTTT	104	60
HPRT	Nygard et al., 2007 [49]	Forward: GGACTTGAATCATGTTTGTG; Reverse: CAGATGTTTCCAAACTCAAC	91	60
OCLN	NM_001163647.2; (NCBI)	Forward: GAGTACATGGCTGCTGCTGA; Reverse: TTTGCTCTTCAACTGCTTGC	102	60
PIGR	NM_214159.1; (NCBI)	Forward: GCCAAGGTCCTGGACAGATA; Reverse: GTACACGGATTTCGGCTTCT	116	60
RPL19	AF_435591; (NCBI)	Forward: AACTCCCGTCAGCAGATCC; Reverse: AGTACCCTTCCGCTTACCG	147	60