Rapamycin Improves Palmitate-Induced ER Stress/NF κ B Pathways Associated with Stimulating Autophagy in Adipocytes

Figure 3

Rapamycin decreases PA-induced inflammatory cytokines and ER stress pathways. Mature 3T3-L1 adipocytes were pretreated with or without RAP (100 nM) for 1 h and with 0.5% BSA or BSA-conjugated PA (0.5 mM) for 12 h. (a, b) mRNA levels of MCP-1 and IL-6 were analyzed by qPCR. (c) Western blot and quantification for the protein expression with the indicated antibodies. (d) IL-6 secretion in mature 3T3-L1 adipocytes medium. Data are presented as mean ± SEM of three to five separate experiments. versus nontreatment group (0.5% BSA); ^# versus PA 0.5 mM group.

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