Astragaloside IV Inhibits NF-κB Activation and Inflammatory Gene Expression in LPS-Treated Mice

Figure 1

AS-IV inhibits LPS-induced increases in serum MCP-1 and TNFα in mice. Mice were randomly assigned to 4 groups as follows: (i) control animals received daily i.p. injection of the vehicle HBSS for 6 days followed by a single i.p. HBSS injection; (ii) AS-IV-treated animals received 10 mg/kg b.w. AS-IV daily i.p. for 6 days followed by a single i.p. HBSS injection; (iii) LPS-treated animals received daily i.p. injection of vehicle HBSS for 6 days followed by a single i.p. injection of 0.5 μg/g b.w. LPS; and (iv) AS-IV plus LPS-treated animals received AS-IV daily i.p. for 6 days followed by single i.p. injection of LPS. Three hours after the HBSS or LPS injection, the animals were sacrificed and blood was collected. Serum MCP-1 (a) and TNFα (b) were measured by ELISA. Data shown are mean values ± SEM of five animals per group. compared to animals treated with LPS only.

(a)

(b)