RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress

Figure 3

RRM2B impacts on mitochondrial homeostasis through regulating mitochondrial genes and copy number under oxidative stress. (a) Cells were treated with H₂O₂ and harvested for Western blot analysis using RRM2B and mitochondrial proteins VDAC1 and COX4 antibodies. GAPDH served as control. Image J was used to normalize VDAC1 and COX4 expression relative to GAPDH, and the normalized figures were shown in 3(a)(ii). (b)(i) H1299 stable cells treated with indicated concentrations of H₂O₂ were harvested 24 hours later for FACS analysis. The detection of MitoTracker was described in Section 2. The intensity of the MitoTracker Green signal relative to untreated control cells is shown here (means ± SEM, ). (b)(ii) Cells were treated with H₂O₂ and harvested at 24-hour time point and underwent FACS analysis. MitoTracker Green probe was used for cell staining, and this figure shows the MitoTracker intensity of the representative data from (b)(i). (c) Cells were treated with 200 μM H₂O₂ and harvested 24 hours later for Q-PCR analysis. Expression of normalized Nd1 relative to untreated control cells is shown (means ± SEM, ).

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