Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Figure 1

Degranulation of eosinophils stimulated with H. pylori OMVs. (a) Freshly isolated human eosinophils were stimulated with H. pylori OMVs (200 μg/mL) for 24 h. Cells were analyzed by TEM (×12,000). OMV-treated cells exhibited cytoplasmic degranulation (arrowhead). (b) Human eosinophils were stimulated with OMVs (200 μg/mL) obtained from wild-type H. pylori or the indicated isogenic mutants for 24 h. The concentrations of ECP in the culture supernatants were measured by ELISA (mean ± SEM, ). control; NS, statistically nonsignificant.

(a)

(b)