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Mediators of Inflammation
Volume 2015, Article ID 308185, 11 pages
http://dx.doi.org/10.1155/2015/308185
Research Article

Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy

1Department of Cardiology, Charité–University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany
2Berlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, Germany
3Medico-Academic Consultings (MEDIACC), 14193 Berlin, Germany
4Department of Cardiology, Charité–University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, Germany
5German Center for Cardiovascular Research (DZHK), 10117 Berlin, Germany
6Institute of Cardiac Diagnostics and Therapy (IKDT), 12203 Berlin, Germany

Received 17 November 2014; Accepted 22 January 2015

Academic Editor: Fulvio D'Acquisto

Copyright © 2015 Caroline Schmidt-Lucke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with or without CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45CD34CD11bCD73+CD90+ cells accounted for 0.010 [0.0025–0.048]%/peripheral mononuclear cell (PMNC) and as CD45CD34CD11bCD73+CD105+ cells for 0.019 [0.0026–0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (, multivariate analysis). A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45CD34CD90+CD105+) in EMB (, ). SDF-1α was the strongest predictor for increased MSC in EMB (, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation.