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Mediators of Inflammation
Volume 2015 (2015), Article ID 361638, 8 pages
http://dx.doi.org/10.1155/2015/361638
Research Article

Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats

1Department of Anesthesiology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan
2School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Road, Hualien 97004, Taiwan
3Department of Pathology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan
4Graduate Institute of Nursing, College of Nursing, Taipei Medical University, 250 Wushing Street, Taipei 11042, Taiwan

Received 3 June 2015; Revised 28 October 2015; Accepted 4 November 2015

Academic Editor: Kazuhiro Ito

Copyright © 2015 Wei-Chi Chou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplmental Figure 1 illustrates the preliminary data of total cell number (A) and protein concentration (B) in bronchoalveolar lavage fluid (BALF). Sham+N/S: the sham-operation plus normal saline group. Sham+C50: the Sham plus 50 mg/kg caffeine group. Sham+C100: the Sham plus 100 mg/kg caffeine group. IR+N/S: the lower limb ischemia-reperfusion plus normal saline group. IR+C50: the IR plus 50 mg/kg caffeine group. IR+C100: the IR plus 100 mg/kg caffeine group. Rats of the Sham+C50, the Sham+C100, the IR+C50 and the I/R+C100 groups received caffeine (intra-peritoneal injection) immediately after reperfusion. To control for the effects of treatment vehicle, rats of the Sham+N/S and the IR+N/S group received normal saline (1.0 mL, intra-peritoneal injection) at the comparable time point. One way analysis of variance with the Bonferroni-Dunn test was used for multiple comparisons. The significance level was set at 0.05. Data were derived from 3 rats from each group and presented as mean ± standard deviation. *P<0.05 the IR+N/S group versus the Sham+N/S group. #P<0.05 the IR+C50 group or the I/R+C100 group versus the IR+N/S group.

  1. Supplementary Material