Cannabinoid Receptor CB2 Is Involved in Tetrahydrocannabinol-Induced Anti-Inflammation against Lipopolysaccharide in MG-63 Cells

Figure 7

The upregulation of cofilin-1 inhibited the THC-induced anti-inflammation. (a) CB2 antagonist reversed the THC-induced effect on cofilin-1 expression. (b) Doxycycline induced an overexpression of cofilin-1 in the MG-63 cells. (c) Doxycycline abolished THC-induced reduction of IL-6 release. (d) Doxycycline abolished the THC-induced reduction of TNF-α release. The cells were treated with different drugs for 24 h (LPS: 10 ng/mL; THC: 5 μM; AM630: 10 μM; doxycycline: 0.1 μg/mL), western blot was performed to evaluate the cofilin-1 expression, and ELISA was used to test the concentrations of IL-6 and TNF-α in the supernatants. Results are means ± SD (). : .

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