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Mediators of Inflammation
Volume 2015 (2015), Article ID 364710, 13 pages
http://dx.doi.org/10.1155/2015/364710
Research Article

Decreased Regulatory T Cells in Vulnerable Atherosclerotic Lesions: Imbalance between Pro- and Anti-Inflammatory Cells in Atherosclerosis

1Department of Internal Medicine I, Friedrich-Schiller-University, 07747 Jena, Germany
2Institute of Forensic Medicine, Friedrich-Schiller-University, 07743 Jena, Germany
3Department of Vascular Surgery, Friedrich-Schiller-University, 07747 Jena, Germany
4Department of Vascular Surgery, Thüringen Klinik Saalfeld, 07318 Saalfeld, Germany

Received 27 July 2014; Accepted 20 October 2014

Academic Editor: Dianne Cooper

Copyright © 2015 Ilonka Rohm et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Atherosclerosis is a chronic inflammatory disease of the arterial wall in which presentation of autoantigens by dendritic cells (DCs) leads to the activation of T cells. Anti-inflammatory cells like Tregs counterbalance inflammation in atherogenesis. In our study, human carotid plaque specimens were classified as stable (14) and unstable (15) according to established morphological criteria. Vessel specimens () without any signs of atherosclerosis were used as controls. Immunohistochemical staining was performed to detect different types of DCs (S100, fascin, CD83, CD209, CD304, and CD123), proinflammatory T cells (CD3, CD4, CD8, and CD161), and anti-inflammatory Tregs (FoxP3). The following results were observed: in unstable lesions, significantly higher numbers of proinflammatory cells like DCs, T helper cells, cytotoxic T cells, and natural killer cells were detected compared to stable plaques. Additionally, there was a significantly higher expression of HLA-DR and more T cell activation (CD25, CD69) in unstable lesions. On the contrary, unstable lesions contained significantly lower numbers of Tregs. Furthermore, a significant inverse correlation between myeloid DCs and Tregs was shown. These data suggest an increased inflammatory state in vulnerable plaques resulting from an imbalance of the frequency of local pro- and anti-inflammatory immune cells.