Research Article
Decreased Regulatory T Cells in Vulnerable Atherosclerotic Lesions: Imbalance between Pro- and Anti-Inflammatory Cells in Atherosclerosis
Table 3
Antibodies used for immunohistochemical analyses.
| Antibodies | Source | Dilution | Specificity |
| CD86 | Milteyi Biotec | 1 : 25 | APCs | CD68 | Dako | 1 : 50 | Macrophages | S-100 | Dako | 1 : 500 | Glial Cells, ependyma, Schwann cells, mDCs | Fascin | Dako | 1 : 100 | mDCs | CD83 | Serotec | 1 : 40 | Mature mDCs | CD209 | BD Pharmingen | 1 : 100 | Immature mDCs | CD304 | Miltenyi Biotec | 1 : 100 | pDCs | CD123 | Serotec | 1 : 100 | pDCs | HLA-DR | Dako | 1 : 25 | APCs | CD3 | Miltenyi Biotec | 1 : 100 | T cells | CD4 | Dako | 1 : 50 | T helper cells | CD8 | Dako | 1 : 200 | Cytotoxic T cells | CD161 | Serotec | 1 : 20 | Natural killer cells | CD25 | Invitrogen | 1 : 20 | Activated B and T cells, regulatory T cells | CD69 | Ab cam | 1 : 100 | Activated B and T cells, regulatory T cells (early activation marker), macrophages, and platelets | FoxP3 | BioLegend | 1 : 20 | Regulatory T cells | CD34 | Dako | 1 : 50 | Endothelial cells | CCR4 | BioLegend | 1 : 50 | Chemokine receptor of DC, T cells, and B cells | CCR-6 | R&D | 1 : 80 | Chemokine receptor of immature DCs |
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APC: antigen presenting cell; mDCs: monocytoid dendritic cells; pDCs: plasmacytoid dendritic cells.
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