Table 1: Recombinant antigens of M. tuberculosis used in this study.

Protein name(s)Rv number Mol mass (kDa)Expression vector 6-fold His-TagE. coli host strain

NarLRv0844c23.9pET22N-terminalBL21 (DE3)
AlaDH Rv278038.7pJLA604CAG629
19 kDa glycolipoprotein, LpqHRv376316.0 pET26C-terminalBL21 (DE3)
PstS3Rv092838.8pET22C-terminalRosetta (DE3)
MPT83, MPB83Rv287324.9pET21C-terminalBL21 (DE3) [pLysS]

Nitrate/nitrite response transcriptional regulatory protein NarL; secreted L-alanine dehydrogenase (AlaDH); 19 kDa lipoprotein antigen precursor LpqH; periplasmic phosphate-binding lipoprotein PstS3; cell surface lipoprotein MPT83.