Mediators of Inflammation / 2015 / Article / Fig 4

Research Article

CXCR6 Expression Is Important for Retention and Circulation of ILC Precursors

Figure 4

Intestinal ILC compartments have heterogeneous expression of CXCR6 and egress markers. (a) Flow cytometry of Lin (Lin: CD3ε, CD5, CD8, CD11c, CD19, TCRβ, TCRγδ, Ter119, and Gr1) adult intestinal LP from Rag2/ Cxcr6Gfp/+ mice. ILC subsets are defined as cNK (filled gray,   NK1.1+  NKp46+ IL-7Rα), ILC1 (blue, Lin NK1.1+ NKp46+ IL-7Rα), ILC2 (red, Lin  NK1.1        ), ILC3 NCR+ (green, Lin NK1.1      c-Kit NKp46+), ILC3 NCR (orange, Lin NK1.1       NKp46+ CD4), and LTi-like (light blue, Lin NK1.1       NKp46+ CD4+). Each compartment is analyzed for CXCR6-GFP expression. (b) Flow cytometry of Lin adult intestinal LP from Rag2/ Cxcr6Gfp/+ mice. ILC subsets are defined as ILC2 (red, Lin GATA-3+ RORγt), ILC3 NCR+ (green, Lin GATA-3 RORγt+ NKp46+ CD4), ILC3 NCR (orange, Lin GATA-3 RORγt+ NKp46 CD4), LTi-like (light blue, Lin GATA-3 RORγt+ NKp46 CD4+), and ILC1-cNK (blue, Lin GATA-3 RORγt NKp46+ NK1.1+). (c) Histograms depicting levels of CD62L, CD69, CD44, CXCR6-GFP, and α4β7 in Lin (filled gray) or ILC subsets (as defined in Figure 3(b)) in adult LP (upper panels) or mesenteric lymph nodes (mLN, lower panels) from Rag2/ Cxcr6Gfp/+ mice. Results are representative of at least 3 experiments each ((a), (b), (c): ).
(a)
(b)
(c)