Punicalagin Induces Nrf2/HO-1 Expression via Upregulation of PI3K/AKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages

Figure 5

Blocking Nrf2 suppresses modulatory effect of PI3K/Akt pathway on PUN-induced HO-1 protein expression. RAW264.7 cells were treated for 8 h with 100 μM PUN in the presence or absence of indicated inhibitor (LY294002, 20 μM; brusatol, 10 nM) and inducer (insulin, 10 μM) at 37°C in a humidified incubator with 5% CO₂. The HO-1 protein expression was analyzed by Western blotting. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. indicates significant differences compared with the PUN-treated group.

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