Downregulation of Lung Toll-Like Receptor 4 Could Effectively Attenuate Liver Transplantation-Induced Pulmonary Damage at the Early Stage of Reperfusion

Figure 3

The effect of TLR4 siRNA on OALT-induced ALI in rats. (a) and (c) TLR4 gene and protein expression during screening for siRNAs. Groups BC, NC, siRNA1, siRNA2, and siRNA3 in A were treated with vehicle, negative control siRNA, siRNA1, siRNA2, and siRNA3, respectively. (b) and (d) showed the effects of TLR4 siRNA on TLR4 protein expression in liver tissue. (b), (e), and (f) showed the effects of TLR4 siRNA on TLR4 mRNA and protein expression in lung tissue. Groups S, NS, NC, and siRNA in (b) to (d) were treated with nothing, saline, negative control siRNA, and targeting siRNA, respectively. Values were expressed as mean ± SEM. NS: no statistical significance; , , one-way ANOVA with Tukey test.