Research Article
Differential Contribution of BLT1 and BLT2 to Leukotriene B4-Induced Human NK Cell Cytotoxicity and Migration
Figure 3
(a) LTB4 enhances NK cell cytolytic function via the BLT1 receptor. PBLs (effector cells) were preincubated without antagonist (■) or with U75302 10 μM (∆) or LY255283 50 μM (□) for 30 minutes at 37°C and then were coincubated with CFSC-labelled K562 cells at E : T = 50 : 1 ratio in the presence of graded concentrations of LTB4. Cytotoxicity was measured using the percentage of 7AAD positive events gating on K562 cells. Each curve represents mean ± SEM of seven independent experiments. , and by one-way ANOVA with Dunnett posttest to controls. , , and by two-way ANOVA with Bonferroni posttests to no-antagonist data. (b) Effects of LTB4 or CAY10583 on NK cell cytotoxicity in the absence or presence of LY255283 or U75302. Data are expressed as means ± SEM of cytotoxicity ratios. using Student’s paired -test; .
| (a) |
| (b) |