Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury

Figure 7

Caspase-3 activation. Changes in the activation of caspase-3 were investigated by immunoblotting of lysates of mononuclear intravascular leukocytes isolated by perfusion of control kidneys and isogenic (Iso) and allogenic (Allo) renal transplants (a) as well as total control and graft tissue lysates (c) on day 42 after transplantation. Detection of the house-keeping enzyme GAPDH was performed for normalization. Densitometric quantification of the bands from perfusate blots is depicted in (b) ( per group). Box plots indicate median and percentiles 0, 25, 75, and 100. . OD: optical density.

(a)

(b)

(c)