Roles of Lymphocyte Kv1.3-Channels in the Pathogenesis of Renal Diseases and Novel Therapeutic Implications of Targeting the Channels

Figure 3

Effects of margatoxin and benidipine on Kv1.3 channel currents in murine thymocytes. Typical whole-cell current traces at different voltage steps recorded before and after the application of 100 nM margatoxin (a) or 10 μM benidipine ((b)(A)). The currents were elicited by voltage-steps from the holding potential of −80 mV to −40, 0, 40, and 80 mV, as depicted in the voltage protocol. Each pulse was applied for a 200-ms duration between 10-second intervals. (b)(B) Peak current densities (peak currents normalized by the membrane capacitance) obtained from the records in (A) at the voltage-step of 80 mV. (b)(C) Pulse-end current densities (pulse-end currents normalized by the membrane capacitance) obtained from the records in (a) at the voltage-step of 80 mV. ^# versus before the drug application. Values are means ± SEM (). Differences were analyzed by ANOVA followed by Dunnett’s or Student’s -test. Modified from [18, 38].

(a)

(b)