Lipid Peroxidation-Mediated Inflammation Promotes Cell Apoptosis through Activation of NF-κB Pathway in Rheumatoid Arthritis Synovial Cells

Figure 4

Activated NF-κB pathway by 4-HNE treatment in MH7A synovial cells. ((a)-(b)) Western blots and histograms showing that the nuclear translocations of NF-κB (p65) are increased by 4-HNE treatment of low concentration (5 μM) for 3 and 12 h. Cyt: cytosol; Nuc: nuclear. ((c)-(d)) Western blots and histograms showing that nuclear translocation and activity of NF-κB (p65) are increased by 4-HNE treatment of high concentration (50 μM) for 3 h and decreased in 12 h. Results are averages of three independent experiments. Data represent mean ± SEM. ^∗, ^∗∗, and ^∗∗∗.

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