Lipid Peroxidation-Mediated Inflammation Promotes Cell Apoptosis through Activation of NF-κB Pathway in Rheumatoid Arthritis Synovial Cells

Figure 6

Inhibition of NF-κB activation reduces inflammation in MH7A synovial cells. ((a)-(b)) Histograms showing mRNA levels of IL1-β are reduced by BAY11-7082 (NF-κB inhibitor) treatment (10 μM) under the condition of 4-HNE treatment of low concentration (5 μM, 0~12 h) (a) and high concentration (50 μM, 0~12 h) (b). Results are averages of five independent experiments. Data represent mean ± SEM. ^∗, ^∗∗, and ^∗∗∗. ((c)-(d)) Western blots showing protein levels of COX-2 are partly reduced by BAY11-7082 treatment (10 uM) under the condition of 4-HNE treatment of low concentration (5 μM, 0~12 h) (c), while not altered in groups of high concentration (50 μM, 0~12 h) (d).

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