Exogenous S1P Exposure Potentiates Ischemic Stroke Damage That Is Reduced Possibly by Inhibiting S1P Receptor Signaling

Figure 1

Schematic representation of experimental protocols. (a) Scheme for Figures 2 and 4 (an initial stroke model). Mice were challenged by 90 min occlusion followed by 22 h reperfusion. FTY720 (3 mg/kg, i.p.) was administered to mice immediately after reperfusion. (b) Scheme for Figure 3. S1P was microinjected into the corpus callosum (CC) and brain samples were prepared 1 day after microinjection. FTY720 was administered 30 min prior to S1P microinjection. (c) Scheme for Figures 5 to 7 (a recurrent stroke-mimicking model). S1P was at first microinjected into the CC. One day later, mice were challenged by 60 min occlusion followed by 22 h reperfusion. FTY720 (3 mg/kg, i.p.) was administered to mice 30 min prior to MCAO.