Exogenous S1P Exposure Potentiates Ischemic Stroke Damage That Is Reduced Possibly by Inhibiting S1P Receptor Signaling

Table 1

Primers used for PCR analysis.

Gene	Direction	Sequence	Gene accession number
β-actin	Forward	5′-AGCCTTCCTTCTTGGGTATG-3′	NM_007393
	Reverse	5′-CTTCTGCATCCTGTCAGCAA-3′
S1pr1	Forward	5′-AGGGAACTTTGCGAGTGAG-3′	NM_007901
	Reverse	5′-GTTACAGCAAAGCCAGGTCAG-3′
S1pr2	Forward	5′-ATAGACCGAGCACAGCCAAC-3′	NM_010333
	Reverse	5′-GTGTTCCAGAACCTTCTCAGG-3′
S1pr3	Forward	5′-TTGCAGAACGAGAGCCTATT-3′	NM_010101
	Reverse	5′-TTCCCGGAGAGTGTCATTTC-3′
S1pr4	Forward	5′-ACCTTCAGTCTGCTCTTCACG-3′	NM_010102
	Reverse	5′-AAGAGCACATAGCCCTTGGAG-3′
S1pr5	Forward	5′-AGATTTCCAATAGCCGCTCTC-3′	NM_053190
	Reverse	5′-AGCTTGCCGGTGTAGTTGTAG-3′
Sphk1	Forward	5′-AGTCATGTCCGGTGATGGTC-3′	NM_011451
	Reverse	5′-CCAGTTGGCCTTGGTAGATG-3′
Sphk2	Forward	5′-ATCTCTGAAGCTGGGCTGTC-3′	NM_203280
	Reverse	5′-GAAGAAGCGAGCAGTTGAGC-3′