Research Article

S-Nitrosoglutathione Reductase Plays Opposite Roles in SH-SY5Y Models of Parkinson’s Disease and Amyotrophic Lateral Sclerosis

Figure 6

Characterization and sensitivity of G93A models to nitrosative stress. Biotin switch assay of total protein-SNOs (a) and analysis of GSNOR activity (b) performed on spinal cord lysates of WT and G93A-SOD1 expressing mice (G93A). (c) Cytofluorimetric analyses of apoptosis upon propidium iodide staining performed on parental SH-SY5Y cells (WT) and G93A-SOD1 mutants (G93A) transfected or not with siRNA against GSNOR (siGSNOR) and treated for 24 h with 400 μM DPTA. Western blots shown are representative of at least independent experiments that gave similar results. Tubulin was selected as loading control. Graphs shown represent the mean of data ± SD of independent experiments, and ; n.s.: not significant.
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