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Mediators of Inflammation
Volume 2015, Article ID 536894, 8 pages
http://dx.doi.org/10.1155/2015/536894
Research Article

Expression of Chemokine Receptors on Peripheral Blood T Cells in Children with Chronic Kidney Disease

1Dialysis Division for Children, Department and Clinic of Pediatrics, SMDZ in Zabrze, SUM in Katowice, Ulica 3 Maja 13/15, 41-800 Zabrze, Poland
2Department of Pediatric Hematology and Oncology, SMDZ in Zabrze, SUM in Katowice, Ulica 3 Maja 13/15, 41-800 Zabrze, Poland
3Department of Pediatric Nephrology, Wrocław Medical University, Ulica Borowska 213, 50-556 Wrocław, Poland
4Department of Microbiology and Immunology, SMDZ in Zabrze, SUM in Katowice, Ulica Jordana 19, 41-808 Zabrze, Poland
5Department of Anesthesiology and Intensive Care, SMDZ in Zabrze, SUM in Katowice, Ulica 3 Maja 13/15, 41-800 Zabrze, Poland

Received 13 September 2014; Revised 18 February 2015; Accepted 27 February 2015

Academic Editor: Gian M. Ghiggeri

Copyright © 2015 Maria Szczepańska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chemokine receptors play a role in leukocyte recruitment, activation, and maintaining effector functions and regulate adaptive immune response and angiogenesis. The study aimed at flow cytometric analysis of T cell subsets with selected surface chemokine receptors (CCR4, CCR5, CCR7, CXCR3, and CXCR4) or receptor combination in peripheral blood of children with chronic kidney disease (CKD) on hemodialysis (HD). The percentage of T lymphocytes with CD8 and combined CD28,CCR7 expression was higher in HD children. The percentage of T lymphocytes expressing CCR7, CD28,CCR7, and CXCR4,CD8 was increased in children on conservative treatment. Total number (tn) of CXCR4+ cells was reduced in children on hemodialysis. The tn of T CXCR3+ cells was lower in children on conservative treatment. During HD the percentage of T CD4+ cells was higher and of T CXCR3+ lymphocytes was lower after HD session as compared to 15 min of session duration. During HD tn of T cells with expression of CCR4, CCR5, CCR7, CXCR3, and CXCR4 was constant. The alteration of chemokine receptors expression in children with CKD occurs early in the development. Diminished expression of CXCR3, CXCR4 on T cells in patients with CKD on HD might result in impaired inflammatory response. Increased CCR7+ T cell percentage could be responsible for the alteration of migration of cells into secondary lymphatic organs.