Postmitotic Expression of SOD1^G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation

Figure 5

C2C12 MLC/ cells exhibit adipogenic features. Real time PCR analysis for Pax7 (a) and Pax3 (b). (c) Upper panel shows Oil Red O staining and phase contrast of control C2C12 and C2C12 MLC/ transfected cells at day 5 in culture. (d) Upper panel shows representative western blot analysis of Plin2 and relative densitometric analysis (lower panel) of the ratio between Plin2 and α-tubulin in C2C12 (white bars) and C2C12 MLC/ (black bars). D0, D2, and D5 referred to days of culture in differentiation condition. Data are represented as mean ± SEM (; ).

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