Epithelium-Specific Ets-Like Transcription Factor 1, ESE-1, Regulates ICAM-1 Expression in Cultured Lung Epithelial Cell Lines

Figure 1

Induction of ESE-1 and ICAM-1 mRNA expression by TNF-α and IL-1β in different cell lines. (a, b) ESE-1 and ICAM-1 mRNA expression in BEAS2B, IB3-1, and A549 cells after stimulation with TNF-α and IL-1β (10 ng/mL each) for 2 hours. The mRNA expression levels were determined by real-time quantitative RT-PCR and the fold of change was based on the mRNA level of non-induced cells. Data were normalized to GAPDH and values were shown in as the mean ± SD, , . Statistics was performed as described in Materials and Methods. (c, d) ESE-1 and ICAM-1 mRNA expression in A549 cells at different time points following cytokine stimulation. Cells were lysed at time points as indicated after TNF-α and IL-1β (10 ng/mL each) stimulation. The fold of change was based on the expression level at time 0 hour. Data were collected and analyzed as described in (a) and (b).

(a)

(b)

(c)

(d)