The Impact of ATRA on Shaping Human Myeloid Cell Responses to Epithelial Cell-Derived Stimuli and on T-Lymphocyte Polarization

Table 1

Relative expression of chemokines secreted by colon epithelial cell lines. Caco2, HT-29, and HCT116 cells were treated with IL-1 or TNF- in the presence or absence of ATRA for 60 min. After removing the cell culture supernatant the cells were washed and replaced with fresh medium and the supernatants were collected after 5 hours and used for the Chemokine Array analysis according to the manufacturer’s instruction to determine the variety of chemokines secreted by these cell lines. The values indicate the results of the densitometric analysis of dot blots and are normalized to the relevant sample control according to the manufacturer’s instructions. For simplicity the value of 1 obtained after dividing with the sample control is referred to as 100.

		CCL19	CCL21	CCL22	Mk	CXCL16	CXCL7	CXCL1	CXCL8	CCL20
C	Caco2	30	30	30	90	70	40	—	—	—
	HT-29	40	30	40	80	90	60	—	—	—
	HCT-116	150	—	—	170	170	—	—	120	—
IL-1	Caco2	—	—	—	100	80	—	120	140	30
	HT-29	—	—	—	80	90	—	100	90	30
	HCT-116	—	—	—	150	160	100	100	100	100
IL-1 + ATRA	Caco2	—	—	—	110	80	50	100	130	—
	HT-29	—	—	—	100	80	40	100	90	—
	HCT-116	—	—	—	160	140	90	110	110	—
TNF-	Caco2	—	—	—	90	60	—	—	—	—
	HT-29	—	—	—	80	60	—	—	—	—
	HCT-116	—	—	—	150	150	—	10	10	—
TNF- + ATRA	Caco2	—	—	—	90	60	—	—	—	—
	HT-29	—	—	—	90	80	20	—	—	—
	HCT-116	—	—	—	150	130	20	10	—	—
ATRA	Caco2	—	—	—	50	30	—	—	—	—
	HT-29	—	—	—	60	20	—	—	—	—
	HCT-116	40	20	30	80	80	—	—	—	—