Research Article

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells

Figure 4

Knockdown of GM-CSF upregulates iNOS and COX-2 expression. (a, b) Western blot analysis of Ca Ski, SiHa, and Hela cells transfected with siRNA targeting the GM-CSF gene to detect the expression of GM-CSF (a), iNOS and COX-2 (b), respectively. (c) Unpaired Student’s -test was used to detect the differences of iNOS and COX-2 expression in different groups. Each experiment was repeated three times. GT: GM-CSF siRNA transfection, VT: scrambled siRNA transfection, and NT: nontransfection versus NT group. Error bars represent mean ± SD.
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