The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains
Expression profiling based on Mouse Antifungal Response RTC Profiler PCR Array. (a) A heat map was generated with 84 genes associated with antifungal immune response and five housekeeping genes, using RT2 Profiler Data Analysis Software version 3.5 with the PAMM 00147Z array panel (SABiosciences). Fold change values were determined after each gene was normalized to the constitutively expressed Gusb (A/J) and B2m (B10.A) genes using the comparative threshold method. AIGs 1, 2, and 3 and AIMs 1, 2, and 3 stand for A/J GM-BMM and M-BMM, respectively, infected with P. brasiliensis in relation to its respective control cells. BIGs 1, 2, and 3 and BIMs 1, 2, and 3 stand for B10.A GM-BMM and M-BMM, respectively, infected with P. brasiliensis in relation to its respective control cells. (b) Venn diagram summarizing the results of differentially expressed genes (; fold change ≥2) between the noninfected and P. brasiliensis infected group of GM- or M-BMM of A/J and B10.A mice strains.
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