Mediators of Inflammation / 2015 / Article / Fig 5

Research Article

Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model

Figure 5

CO inhibits TN-C-mediated inflammation a septic mouse model. Wild type seven-week-old male C57BL/6 mice were pretreated with CORM-2 (30 mg/kg, i.p.) or RuCl3 (30 mg/kg, i.p.) for 2 h and then mice were injected with LPS (10 mg/kg, i.p.) for 2 h. Liver tissues were analyzed for mRNA levels of TN-C (a) and TNF-α and IL-6 (b) by Real Time RT-PCR. Blood serum was analyzed for protein expression of TNF-α, IL-6 (c). IL-10 mRNA (d) by Real Time RT-PCR and IL-10 protein (e) by ELISA were measured. The protein levels of TN-C from serum were measured by Western blotting (f). Loading control was detected by ponceau staining. The relative signal intensity of bands was determined and standardized using ImageJ software. Data represents mean ± SEM, , and when compared with control; , , and ns, nonsignificant, as compared with the cells exposed to LPS alone.

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