Research Article

CCR9 Antagonists in the Treatment of Ulcerative Colitis

Figure 3

CCL25 protein levels are significantly elevated during colonic inflammation. CCL25 protein levels in homogenates of colon and terminal ileum were determined by ELISA and normalized to the levels of total protein. (a) CCL25 protein levels were significantly elevated in colon biopsies from individuals with active Crohn’s disease or ulcerative colitis, relative to healthy controls. In Crohn’s disease, the levels of CCL25 in the colon were equivalent to the levels in the terminal ileum. = 6–8 individuals. (b) CCL25 levels were increased in presymptomatic mdr1a−/− mice and further increased in mdr1a−/− mice with severe disease, compared to wild-type mice. = 6–8 mice. (c) The frequency of CCR9+CD4+ T cells among PBMC is significantly increased in mdr1a−/− mice compared to age-matched wild-type mice; = 5 mice per time point. < 0.05; < 0.01.
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