Research Article
Modulation of Extracellular ATP Content of Mast Cells and DRG Neurons by Irradiation: Studies on Underlying Mechanism of Low-Level-Laser Therapy
Table 2
The response of extracellular ATP contents to laser irradiation in presence of selected reagents.
| | Reagents (concentration) | Action | [ATP]o in Irr/[ATP]o in control | Conclusion |
| | NEM (100 μM) | Exocytosis blocker | 0.96 ± 0.08 () | No [ATP]o increased once exocytosis was blocked | | BAPTA-AM (25 μM) | [Ca2+]i chelating agent | 1.01 ± 0.13 () | No [ATP]o increased once [Ca2+]i was chelated | | ARL67156 (100 μM) | Nonspecific ecto-ATPases inhibitor | 0.9 ± 0.02 ()* | Inhibition of [ATP]o was greatly attenuated when the ecto-ATPases were inhibited | ATP standards
(10 pmoles) | — | 1.02 ± 0.06 () | Extracellular ATP was not affected by laser irradiation |
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Data are averages ± SEM. Values are relative to control.
[ATP]o presents extracellular ATP levels.
* < 0.05, compared to the control.
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