Research Article

IL-1 Receptor Blockade Alleviates Graft-versus-Host Disease through Downregulation of an Interleukin-1-Dependent Glycolytic Pathway in Th17 Cells

Figure 1

IL-1β controls the glycolysis pathway and differentiation into T helper (Th) 17 cells. Splenocytes from C57BL/6 mice were activated in the Th0 condition in the presence or absence of either IL-1 receptor antagonist (IL-1Ra) or recombinant IL-1β for 3 days. (a) The concentrations of interferon- (IFN-) γ, IL-4, and IL-17 in culture supernatants were measured by means of enzyme-linked immunosorbent assays (ELISAs). ((b) and (c)) The mRNA levels of IL-17, RORγt, and glycolysis-related factors were quantified using real-time PCR. Splenocytes from C57BL/6 mice were cultured under Th17-polarizing conditions for 3 days in the presence or absence of IL-1Ra. (d) The proportion of CD4+IL-17+ cells or CD4+CD25+Foxp3+ cells was determined using intracellular flow cytometric analysis. (e) The mRNA expression of IL-17, IL-21, Runx1, RORγt, Glut1, HK2, GPI, and MNOL was quantified using real-time PCR. , , and . Data are representative of 2 independent experiments.
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