Prostacyclin Synthase: Upregulation during Renal Development and in Glomerular Disease as well as Its Constitutive Expression in Cultured Human Mesangial Cells

Table 1

Prostanoid formation of cytokine-stimulated HMC from different concentrations of exogenously added arachidonic acid.

AA (0 M)	242 ± 42^***	78 ± 8^***	96 ± 7^ns	40 ± 13^ns
AA (2 M)	6590 ± 300^***	6120 ± 370^***	110 ± 7^ns	560 ± 6.6^ns
AA (5 M)	11350 ± 250^***	10390 ± 610^***	83 ± 7^ns	116 ± 7^ns
AA (20 M)	14800 ± 100^***	13500 ± 200	100 ± 24^ns	226 ± 40

HMC were stimulated with cytokines for 20 h and later they were incubated with the depicted concentrations of exogenous arachidonic acid (AA) or only buffer (0 M) for 15 min at 20°C. Prostaglandins generated were extracted from the supernatant as described and analyzed by GC/MS/MS method. Values are depicted as means ± SEM (pg/10⁶ cells) of three experiments. One-way analysis of variance Bonferroni’s multiple comparison test. < 0.001 for all combinations. < 0.01 for 5 M versus 20 M; < 0.01 for 0 M versus 20 M; < 0.01 for 2 M versus 20 M, and < 0.05 for 5 M versus 20 M; ns: not significant.