Figure 1: Expression of sEH in kidney is increased after unilateral ureteral obstruction (UUO) surgery. (a) Eight-week-old WT mice received UUO surgery. In 14 days after UUO, kidneys were harvested and tissue lysates were subjected to western blot to examine the protein levels of sEH and GAPDH. (b) Kidney specimens from mice were immunostained with anti-IgG or anti-sEH antibody and then recognized by corresponding horseradish peroxidase-conjugated secondary antibody. Hematoxylin was used for counterstaining. Scalar bar: 50 μm. Data are mean ± SEM from 8 mice. versus WT nonobstructed kidney.