Figure 4: Deficiency of sEH reduces the leukocyte infiltration and cytokine production. ((a) and (b)) 14 days after UUO surgery, kidneys were harvested. Kidney lysates from WT and sEH−/− mice were immunoblotted with F4/80, CD3, MPO, inducible nitric oxide synthase (iNOS), and GAPDH. (c) The levels of MCP-1, MIP-2, IL-1β, and IL-6 in kidneys were assessed by ELISA kits. Data are mean ± SEM from 8 mice. versus WT nonobstructed kidney; versus WT UUO kidney.