Research Article
EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection
Figure 2
IL-28A inhibits assembly and release of infectious HCV particles. ((a) and (c)) Huh7.5 cells were infected with HCV (MOI = 0.1) for 6 h at 37°C. The medium was then removed and cells were incubated with IL-28A for 48 or 72 h at 37°C. The intracellular virus particles (a) and the cell culture supernatants (c) were harvested and titered by FFU (focus-forming unit) assay on naive Huh7.5 cells. (e) HCV RNA in the cell culture supernatants was extracted and detected with real-time PCR. ((b), (d), and (f)) The dose-response curves of IL-28A. (b) The intracellular virus particles were detected as described in part (a). (d) The secreted infectious virus particles were detected as described in part (c). (f) HCV genome equivalents were detected as described in part (e). The results are the average of three independent experiments performed in triplicate.
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