EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection

Figure 3

IL-28A and IFN-α combination synergistically inhibits HCV replication and induces ISGs expression. (a) Huh7.5 cells were infected with HCV (MOI = 0.1) for 6 h at 37°C. The medium was then removed and cells were incubated with IL-28A or IFN-α or together for 48 h at 37°C. HCV NS5A and core proteins were detected by western bolt. (b) Huh7.5 cells were treated by IL-28A or/and IFN-α for different time courses. The expressions of ISGs, including MX1, OAS1, EPSTI1, APOL6, TRIM69, 1-8U, IFI44L, IFIT1, IRF9, CD274, and CMPK2, were determined by real-time PCR and normalized with GAPDH.

(a)

(b)