EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection

Figure 6

The inhibition of HCV triggered by EPSTI1 is dependent on PKR. ((a) and (b)) Huh7.5 cells (a) and HLCZ01 cells (b) were infected with HCV (MOI = 0.1). EPSTI1 mRNA was detected with real-time PCR and normalized with GAPDH. ((c), (d), and (e)) Huh7.5 and HLCZ01 cells were transfected with p3XFLAG-CMV-EPSTI1 or pSilencer-EPSTI1 followed by HCV infection. Intracellular HCV RNA and PKR mRNA were detected by real-time PCR and normalized with GAPDH. The proteins of PKR and p-PKR were detected by western blot. ((f) and (g)) p3XFLAG-CMV-EPSTI1 or/and pSilencer-PKR was delivered into HLCZ01 cells followed by HCV infection. (f) HCV RNA and PKR mRNA were detected by real-time PCR and normalized with GAPDH. NS5A and PKR proteins were detected by western blot. (g) IFN-β, IFIT1, OAS1, and RNASE L mRNA were detected by real-time PCR and normalized with GAPDH.

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