(a)
(b)
Figure 6: mRNA levels assessed by quantitative PCR of (a) villin using -actin as housekeeping gene and (b) LFABP and IFABP using villin as housekeeping gene. Quantitative PCR analysis was performed in whole duodenal biopsies from adult and pediatric populations of healthy non-CD controls and CD patients at diagnosis. mRNA relative levels from adults and pediatric patients were plotted together since no differences between both populations were observed. (a) mRNA levels of villin using -actin as housekeeping gene. Pediatric samples: controls (), CD patients (). Adult samples: controls (), CD patients (). (b) mRNA levels of LFABP and IFABP using villin as housekeeping gene. Pediatric samples: controls (), CD patients (). Adult samples: controls (), CD patients (). Results were plotted as relative unit (RU). Values are expressed as mean ± standard deviation (s.d.) of the mean. Representative experiments were analyzed statistically using the Mann-Whitney test. (A) ; (B) .